Accurate and fast measurement of catecholamines and other monoamine neurotransmitters
in various biological matrices presents numerous difficulties. This is due to: 1)
the low physiologic concentrations of catecholamines and other monoamine neurotransmitters
(typically in the sub ng/mL range); 2) the tendency of the catechol group to be
oxidized; 3) relatively small sample volumes collected from preclinical models;
and 4) potential interference from drugs and drug metabolites that are structurally
similar to catecholamines and other monoamine neurotransmitters. It has been reported
that reductive ethylation labeling of amino groups of catecholamines and other monoamine
neurotransmitters affords 16 - 100 times increased detection sensitivity of corresponding
native counterparts during the UPLC-MS/MS analysis. Therefore, it enables the use
of a UPLC/MS/MS-based method for simultaneous measurement of catecholamines and
other monoamine neurotransmitters in various biological matrices. We offer a complete
and easy to conduct reductive ethylation labeling kit to enable you to reproduce
these assays in your lab.
Chengjie Ji, Wenlin Li, Xiao-dan Ren, Ayman F El-Kattan, Rouba Kozak, Scott Fountain
and Christopher Lepsy “Diethylation Labeling Combined with UPLC/MS/MS for Simultaneous
Determination of a Panel of Monoamine Neurotransmitters in Rat Prefrontal Cortex
Microdialysates” Anal. Chem. 2008, 80, 9298–9309.
Chengjie Ji, Justin Walton, Yi Su and Max Tella, “Simultaneous Determination of
Plasma Epinephrine and Norepinephrine Using an Integrated Strategy of a Fully Automated
Protein Precipitation Technique, Reductive Ethylation Labeling and UPLC-MS/MS”,
Analytica Chimica Acta 2010, 670, 84-91.
G. Zhang, Y. Zhang, C. Ji, T. McDonald, J. Walton, E.A. Groeber, R.C. Steenwyk,
and Z. Lin, Ultra sensitive measurement of endogenous epinephrine and norepinephrine
in human plasma by semi-automated SPE-LC-MS/MS. J. Chromatogr. B Analyt. Technol.
Biomed. Life Sci. 2012, 895-896 186-90.