Reductive Ethylation Labeling Kit

complete and easy
Accurate and fast measurement of catecholamines and other monoamine neurotransmitters in various biological matrices presents numerous difficulties. This is due to: 1) the low physiologic concentrations of catecholamines and other monoamine neurotransmitters (typically in the sub ng/mL range); 2) the tendency of the catechol group to be oxidized; 3) relatively small sample volumes collected from preclinical models; and 4) potential interference from drugs and drug metabolites that are structurally similar to catecholamines and other monoamine neurotransmitters. It has been reported that reductive ethylation labeling of amino groups of catecholamines and other monoamine neurotransmitters affords 16 - 100 times increased detection sensitivity of corresponding native counterparts during the UPLC-MS/MS analysis. Therefore, it enables the use of a UPLC/MS/MS-based method for simultaneous measurement of catecholamines and other monoamine neurotransmitters in various biological matrices. We offer a complete and easy to conduct reductive ethylation labeling kit to enable you to reproduce these assays in your lab.

Reference :

Chengjie Ji, Wenlin Li, Xiao-dan Ren, Ayman F El-Kattan, Rouba Kozak, Scott Fountain and Christopher Lepsy “Diethylation Labeling Combined with UPLC/MS/MS for Simultaneous Determination of a Panel of Monoamine Neurotransmitters in Rat Prefrontal Cortex Microdialysates” Anal. Chem. 2008, 80, 9298–9309.

Chengjie Ji, Justin Walton, Yi Su and Max Tella, “Simultaneous Determination of Plasma Epinephrine and Norepinephrine Using an Integrated Strategy of a Fully Automated Protein Precipitation Technique, Reductive Ethylation Labeling and UPLC-MS/MS”, Analytica Chimica Acta 2010, 670, 84-91.

G. Zhang, Y. Zhang, C. Ji, T. McDonald, J. Walton, E.A. Groeber, R.C. Steenwyk, and Z. Lin, Ultra sensitive measurement of endogenous epinephrine and norepinephrine in human plasma by semi-automated SPE-LC-MS/MS. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2012, 895-896 186-90.