Differential Dimethyl Labeling kit

complete and easy
Although the strategic use of enzymatic digestion, combined with isotope dilution mass spectrometry, has been increasingly developed and used for the absolute quantification of therapeutic and endogenous proteins in the biopharmaceutical industry over the past several years, the lack of an appropriate internal standard has become the rate limiting step in the development of a standardized analytical approach to provide bioanalytical support for both preclinical and clinical studies. We have reported a universal strategy for the rapid development of a simple, accurate, precise, and high throughput LC-MS/MS-based method for absolute quantification of mAb using differential dimethyl labeling coupled with UPLC-MS/MS. Differential dimethyl labeling provides an easy, fast and cost-effective way to generate stable isotope labeled internal standards (IS) which enables, within 1-2 weeks, the rapid development of an LC-MS/MS-based assay applicable to both preclinical and clinical studies. We offer a complete and easy to conduct differential dimethyl labeling kit to enable you to facilitate your quantitative protein assay development and implementation.

Reference :

Chengjie Ji, Nalini Sadagopan, Yizhong Zhang and Christopher Lepsy “A Universal Strategy for Fast Development of a Method for Absolute Quantification of Therapeutic Monoclonal Antibodies in Biological Matrices Using Differential Dimethyl Labeling Coupled with UPLC-MS/MS” Anal. Chem. 2009, 81, 9321-9328.