ADMET Targets Quantification

quantitative ADMET targets using UPLC-MRM MS assays

In recent years, a MRM mass spectrometry platform has been developed and applied for quantitative measurement of ADMET targets in transfected cells and various tissues to support drug discovery and development.

However, reliable and reproducible quantification of membrane transporters in various biological matrices remains a difficult analytical challenge due to the lack of purified protein standards and the intrinsic hydrophobic property of membrane proteins.

We can quickly develop and implement a highly sensitive, cost-effective UPLC-MRM MS assay for reproducible and reliable quantification of one or multiple membrane transport proteins in cells and various tissues from different species using our extensive expertise and experience in differential dimethyl labeling, and membrane protein isolation, solublization and digestion. We can provide high quality data for your new drug discovery and development, and for your publications.

We offer bioanalyses of the following membrane transporters in different biological matrices (various tissues, hepatocytes and other cells) using our optimized membrane protein extraction, solubilization and digestion protocol:

  • Human: BCRP, PgP, BSEP, MRP1,MRP2, MRP4, MRP5, OATP1B1, OATP1B3, OATP2B1, OATP1A2;
  • Mouse: BCRP, PgP, BSEP, MRP1, MRP2, MRP4, MRP5, SO1A1, SO1A4, SO1B2;
  • Rat: BCRP, PgP, BSEP, MRP1, MRP2, MRP5, OATP1, OATP2, OATP4.

Reference :

Chengjie Ji, William R. Tschantz, Nathan D. Pfeifer, Mohammed Ullah, Nalini Sadagopan, “Development of a multiplex UPLC-MRM MS method for quantification of human membrane transport proteins OATP1B1, OATP1B3 and OATP2B1 in in vitro systems and tissues” Analytica Chimica Acta 2012, 717 67-76