Protein glycosylations are among the most abundant protein post translational modifications, and can have major impacts on protein stability, function and immunogenicity (in the form of non-mammalian glycans). Regulatory agencies scrutinize biologics products with constitutive glycosylation according to the ICH Q6B, “For glycoproteins, the carbohydrate content (neutral sugars, amino sugars, and sialic acids) is determined. In addition, the structure of the carbohydrate chains, the oligosaccharide pattern (antennary profile) and the glycosylation site(s) of the polypeptide chain is analyzed, to the extent possible”